Kinetic Studies on Yeast Growth (part 1)

Kinetic studies of yeast hexokinase.

The mechanism of calf brain hexokinase has recently been studied in our laboratory (1). It was concluded from kinetic experiments that adenosine diphosphate (ADP) dissociates from the enzyme before addition of substrate glucose. This hypothesis was supported by kinetic studies of n-mannose, ADP, and glucose g-phosphate inhibition. The experiments appear to lend credence to theories in which an ...

Studies of the kinetic mechanism of orotate phosphoribosyltransferase from yeast.

Kinetic analysis of the reaction catalyzed by a homogeneous preparation of orotate phosphoribosyltransferase from yeast has revealed that the reaction may proceed through the use of a Bi Bi Ping Pong kinetic mechanism. This interpretation is based on initial rate measurements and product inhibition studies of both the forward phosphoribosyl transfer and the reverse pyrophosphorolysis reactions ...

Studies on Growth Acceleration in Protozoa and Yeast

Structural and kinetic studies on metallo-β-lactamase IMP-1.

In an effort to probe for metal binding to metallo-β-lactamase (MβL) IMP-1, the enzyme was overexpressed, purified, and characterized. The resulting enzyme was shown to bind 2 equiv of Zn(II), exhibit significant catalytic activity, and yield EXAFS results similar to crystallographic data previously reported. Rapid kinetic studies showed that IMP-1 does not stabilize a nitrocefin-derived reacti...

Li ion kinetic studies on spinel cathodes , Li ( M 1 /

Pristine and substituted spinels, Li(M1/6Mn11/6)O4 (M ~ Co1/6, Co1/12Al1/12) were prepared and their cathodic performance up to 80 cycles at ambient temperature and at 50 uC and the Li ion kinetic parameters obtained by galvanostatic intermittent titration technique (GITT) at ambient temperature and 50 uC and electrochemical impedance spectroscopy (EIS) at ambient temperature up to 80 cycles we...